FURTHER STUDIES ON VITAMINS Blo AND Bn AND THEIR RELATION TO “FOLIC ACID” ACTIVITY”

A previous report from this laboratory (1) demonstrated the existence of two water-soluble vitamins needed by the chick; namely, vitamin Blo, essential for proper feather formation, and vitamin B11, necessary for growth. Both of these vitamins, present in a liver concentrate (Super Filtrol eluate), were shown to be distinct from “folic acid”l which was also present in the liver concentrate. All three of these factors were adsorbed and eluted from norit and Super Filtrol but were partially separated by fractional precipitation with ethanol. This paper presents further progress toward the separation and isolation of vitamins Blo and Bn and gives additional information on their chemical properties as they exist in impure concentrates. The relationship of these substances to “folic acid” is pointed out.